One of the esteemed ancient surviving structures of ancient Iran is the ritual city of “Persepolis,” which has preserved on its body the biological and semiotic features of the Achaemenid era. Rock reliefs are considered a valuable part of the architecture of this city-founded structure. Today, graphic branding based on the reliefs and statues of this magnificent historical structure is regarded as one of the thriving arts. Preserving the aforementioned symbols and signs and employing the etymological core and semantic content can identify Iranian symbology. On this basis, the present research has sought, by using Aby Warburg’s approach in artistic genealogy, to trace the origin and analyze the language of sign and sense in the body of this ancient phenomenon, and through the semiology and symbology of these themes in Achaemenid culture, to examine their translatability in the form of graphic art. Since Warburg’s approach is based on visual analysis, valuation of images, and evolution through time, this research seeks to find an answer to the question of how the Achaemenid motifs of Persepolis have influenced the cultural-linguistic structure of the ideology of signs. The analysis of frequency-value data also is consistent with and corroborates the findings obtained via Warburgian analysis. The prominent presence of natural motifs and flowers, as symbols of fertility, abundance, and cosmic order, represents power and superiority. Geometric patterns symbolize order and precision, and their structure represents organized organization within the then bureaucratic system. Motifs such as the silver cup and symbols such as the wheat ear, which represent prosperity and public comfort, enjoy much value in Achaemenid rule. The role of Achaemenid art in designing national symbols and commercial logos shows the lasting impact of the Achaemenid visual language in contemporary Iran. The semiological revitalization of traditional symbols and the preservation of their connection with the heritage of the past is one of the valuable lessons that can be learned via the pro-translation study of Achaemenid art.